Anti-ARP8 | Actin-related protein 8, (clone 12F5,D4,C3), C-Terminal

• Background:

  Actin-related proteins (ARPs)are found in the nuclei of all eukaryotic cells, but their functions are generally understood only in the context of their presence in various yeast and animal chromatin-modifying complexes. Arabidopsis ARP8 shows 30 and 29% amino acid identity to yeast actin and Arabidopsis ACT2 in the regions of alignment, respectively. Because it is not closely related to yeast or human ARP8 and shows similar weak homology to yeast ARP8 and ARP9, the Arabidopsis ARP8 is considered a plant-specific orphan ARP.

• Host:

  Mouse

• Reactivity:

  Arabidopsis thaliana

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  MAP- conjugated peptide ofArabidopsis thalianaARP8, C-terminal UniProt:Q9FKT0-1, TAIR:At5g56180

• Clonality:

  Monoclonal

• Applications:

  ELISA (ELISA), Immunocytochemistry (ICC), Immunoflourescence (IF), Western blot (WB)

• Dilution:

  assay dependent

• Purity:

  Cell culture supernatant

• Format:

  Liquid

• Molecular Weight:

  52 kDa

• Precautions:

  Antibody is recognizing following epitope, amino acids 447-471: SNLSIFPGPWCITRKQFRRKSRLMWThis antibody is recognizing the full-length 52 kDa recombinant ARP8 protein expressed inEscherichia colias well as endogenous ARP8 of identical molecular weight inArabidopsis thalianaextracts from different tissues: all vegetative and reproductive organs examined including seedlings, roots and siliques, with higher concentrations of the protein detected in  developing flower buds and flowers within the inflorescence.

• References & Citations:

  Kandasamyet al. (2008). ACTIN-RELATED PROTEIN8 encodes an F-box protein localized to the nucleolus in Arabidopsis. Plant Cell Physiol. 49(5):858-63. doi: 10.1093/pcp/pcn053.

• Storage Conditions:

  Store at -20°C; Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4