Anti-GLN2 | GS2, chloroplastic form of glutamine synthetase

Anti-GLN2 | GS2, chloroplastic form of glutamine synthetase

• Background: Glutamine synthetase(GLN or GS) is one of the key enzymes involved in nitrogen metabolism of plants. It catalyses the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. There are two general classes of glutamine synthetase in plants: GLN1, a cytosolic form and GLN2, a chloroplastic form. GLN2 is encoded by a single gene and is highly abundant in mesophyll cells of leaves for the assimilation of ammonia produced from photorespiration and the reduction of nitrate in the chloroplasts. GLN2 is a target for thioredoxin.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinacia oleracea
• Not reactive in: Diatoms
• Immunogen: KLH-conjugated synthetic peptide which is a part of part of the glutamine synthetase/guanido kinase superfamily catalytic region chosen from various available sequences, includingArabidopsis thalianaGLN2, UniProt:Q43127, TAIR:AT5G35630
• Clonality: Polyclonal
• Applications: ImmunoGold (IG), (Western blot (WB)
• Dilution: 1 : 5000 on 0.5-5 µg protein/lane detection (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 47 | 44-45 kDa
• References & Citations: Changet al. (2023). Chloroplast import motor subunits FtsHi1 and FtsHi2 are located on opposite sides of the inner envelope membrane. PNAS. 2023 Sep 12;120(37):e2307747120.doi: 10.1073/pnas.2307747120. Epub 2023 Sep 5.Hertleet al. (2021). Horizontal genome transfer by cell-to-cell travel of whole organelles. Science Advances. Volume 7. 01 Jan 2021 : eabd8215 DOI: 10.1126/sciadv.abd8215Ancinet al. (2021) Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco. J Exp Bot. 2021 Jun 22;72(13):4949-4964. doi: 10.1093/jxb/erab193. PMID: 33963398; PMCID: PMC8219043.Chenet al. (2018). TIC236 links the outer and inner membrane translocons of the chloroplast. Nature. 2018 Dec;564(7734):125-129. doi: 10.1038/s41586-018-0713-y.Tamburinoet al. (2017). Chloroplast proteome response to drought stress and recovery in tomato (Solanum lycopersicum L.). BMC Plant Biol. 2017 Feb 10;17(1):40. doi: 10.1186/s12870-017-0971-0.Dixit(2015). Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves. Sci Rep. 2015 Nov 10;5:16205. doi: 10.1038/srep16205.Leeet al. (2013). Stromal protein degradation is incomplete in Arabidopsis thaliana autophagy mutants undergoing natural senescence. BMC Res Notes, Jan 17.Huand Li (2012). The amino-terminal domain of chloroplast Hsp93 is important for its membrane association and functions in vivo. Plant Physiol. Apr;158(4):1656-65. doi: 10.1104/pp.112.193300. Epub 2012 Feb 21.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.