Anti-PYK10 | Beta-Galactosidase (internal) (ER marker, immunolocalisation)

Anti-PYK10 | Beta-Galactosidase (internal) (ER marker, immunolocalisation)

• Background: PYK10is the main component of ER bodies. It has hydrolase activity, hydrolyzing O-glycosyl compounds It may produce defense compounds when plants are damaged by insects or wounding.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana
• Not reactive in: Triticum aestivum
• Immunogen: Conjugated peptide, derived fromArabidopsis thalianainternal part of PYK10,UniProt:A0A178VCN3, TAIR:At3g08880.
• Clonality: Polyclonal
• Applications: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
• Dilution: 1: 100 -1: 500 (IF), 1: 500 (IHC), 1: 500 (IP), 1: 5000 - 1: 20 000 (WB)
• Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
• Format: Liquid at 2 mg/mL.
• Molecular Weight: 59.7 kDa
• Precautions: N-terminal signal peptide including 24 amino acis and ER retention signal is removed from the mature protein
• References & Citations: Yamadaet al. (2013). Identification of Two Novel Endoplasmic Reticulum Body-Specific Integral Membrane Proteins. Plant Physiol. 2013 Jan;161(1):108-20. doi: 10.1104/pp.112.207654. (Western blot, Arabidopsis thaliana)Naganoet al. (2005). Activation of an ER-body-localized ß-Glucosidase via a Cytosolic Binding Partner in Damaged Tissues of Arabidopsis thaliana. Plant Cell Physiol. 2005 Jul;46(7):1140-8. doi: 10.1093/pcp/pci126. (Immunoprecipiation, Western blot, Arabidopsis thaliana)Matshushimaet al. (2003). A novel ER-derived compartment, the ER body, selectively accumulates a beta-glucosidase with an ER-retention signal in Arabidopsis. Plant J . 2003 Feb;33(3):493-502. doi: 10.1046/j.1365-313x.2003.01636.x. Immunofluorescence, Immunohistochemistry, Western blot, Arabidopsis thaliana)
• Storage Conditions: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.