Anti-Rhamnogalacturonan-I backbone (clone CCRC-M35)

Anti-Rhamnogalacturonan-I backbone (clone CCRC-M35)

• Background: Rhamnogalacturonans (RGs)are pectic polysaccharides found in the cell wall, which contain a repeating dissacharides backbone:α-D-GalpA-(1,2)-α-L-Rhap-(1)The antibody was raised against seed mucilage/MeBSA complex and binds to Rha-(1,4)-GalA-(1,2)-Rha-(1,4)-GalA-(1,2)-Rha-(1,4) pectic backbone.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: Arabidopsis thaliana
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: MeBSA-conjugatedArabidopsis thalianaseed mucilage (Rhamnogalacturonan I), non-covalent complex. Epitope structure for carabohydrate antigen: Rha-(1,4)-GalA-(1,2)-Rha-(1,4)-GalA-(1,2)-Rha-(1,4).
• Clonality: Monoclonal
• Applications: ELISA (ELISA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Dilution: Undiluted or at 1 : 10 (ELISA), (IHC), (IF)
• Purity: Cell culture supernatant.
• Format: Liquid
• Precautions: CCRC-M35 binds to the backbone of rhamnogalacturonan I and requires at least two unbranched disaccharide repeats for binding, CCRC-M35 does not bind to branched sections of the backbone and is not sensitive to the identity of the sugar at the non-reducing terminus
• References & Citations: Pattathilet al. (2012). Immunological approaches to plant cell wall and biomass characterization: Glycome Profiling. Methods Mol Biol. 2012;908:61-72.doi: 0.1007/978-1-61779-956-3_6.Patathilet al. (2010). A comprehensive toolkit of plant cell wall glycan-directed monoclonal antibodies. Plant Physiol. 2010 Jun;153(2):514-25.doi: 10.1104/pp.109.151985.
• Storage Conditions: Antibody can be stored up to 1 month at 4°C, and at -80°C for up to 1 year. Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.