Anti-PR-2 | GLU I | Class I beta-1,3-glucanase

Anti-PR-2 | GLU I | Class I beta-1,3-glucanase

• Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known.  Glucanase I  and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1,3-glucanase
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevilandii, Nicotiana glutinosa, Nicotiana tabacum, PhalenopsisSogo Yukidian cultivar V3, Populus sp., Solanum lycopersicum, Solanum tuberosum,Vitis vinifera
• Not reactive in: Arabidopsis thaliana
• Immunogen: Purified tobacco class I, basic  ß-1,3-glucanase. Purified GLU I consists of a mixture of closely related polypeptides encoded by a family of GLU I genes comprising GLAB5APL3derived from the sylvestris ancestor of tobacco, GLBP27666derived from the tomentosiformis ancestor of tobacco and homeologous recombinants (Sperisen et al., 1991). Mature GLU I is processed from a pre-pro-polypeptide (Shinshi et al., 1988).
• Clonality: Polyclonal
• Applications: Immunolocalization (IL), Western blot (WB)
• Dilution: 8 µg/mL (WB)
• Purity: Total IgG in PBS pH 7.4. (without Ca++).
• Format: Lyophilized
• Reconstitution: For reconstitution add 100 µl of sterile water
• Molecular Weight: 37 | 33 kDa
• Precautions: Important note: for blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.
• References & Citations: Liet al. (2021) Penicillium chrysogenum polypeptide extract protects Nicotiana benthamiana against TMV infection through modulation of ABA biosynthesis and callose priming. J Exp Bot. 2021 Mar 4:erab102. doi: 10.1093/jxb/erab102. Epub ahead of print. PMID: 33687058. (Immunolocalization)Colmanet al. (2019). Chitosan microparticles improve tomato seedling biomass and modulate hormonal, redox and defense pathways. Plant Physiology and Biochemistry. Volume 143, October 2019, Pages 203-211.Martin-Saladanaet al. (2018). Salicylic acid loaded chitosan microparticles applied to lettuce seedlings: Recycling shrimp fishing industry waste. Carbohydrate Polymers Volume 200, 15 November 2018, Pages 321-331.Wanget al. (2014). Elicitation of Hypersensitive Responses in Nicotiana glutinosa by the Suppressor of RNA Silencing Protein P0 from Poleroviruses. Mol Plant Pathol. 2014 Sep 4. doi: 10.1111/mpp.12201.Huey-wenet al. (2014). Harpin Protein, an Elicitor of Disease Resistance, Acts as a Growth Promoter in Phalaenopsis Orchids. Journal of Plant Growth Regulation May 2014.Mungeret al. (2012). Beneficial ‘unintended effects’ of a cereal cystatin in transgenic lines of potato, Solanum tuberosum. BMC Plant Biol. 2012 Nov 1;12:198. doi: 10.1186/1471-2229-12-198.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.