Anti-V5 (clone V5,E10)

Anti-V5 (clone V5,E10)

• Background: V5-tagis a tag that can be added to a protein of interest as a fusion protein to enable purification and detection.
• CAS Number: 9007-83-4
• Host: Mouse
• Reactivity: V5-tagged fusion proteins
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated GKPIPNPLLGLDST V5 synthetic peptide
• Clonality: Monoclonal
• Applications: Dot blot (Dot), ELISA (ELISA), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western blot (WB)
• Dilution: 1 : 500-1 :2000 (IHC) , 1 : 1000-1: 3000 (WB)
• Purity: Total IgG. Protein A purified from culture supernatant of hybridoma cells grown in a bioreactor. Provided in 10 mM PBS, pH 7.2, with 1% BSA and 0.05% sodium azide.
• Format: Liquid
• Reconstitution: For reconstitution add 50 µl of sterile water
• Precautions: Antibody is purified on Protein A.For western blot incubate antibody for one hour at room temperature.
• Storage Conditions: Lyophilized antibody can be stored at -20°C for up to 3 years. Re-constituted antibody can be stored at 4°C for several days to weeks. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.