Anti-RPB1 | RNA polymerase II subunit B1

Anti-RPB1 | RNA polymerase II subunit B1

• Background: DNA-directed RNA polymerase II subunit 1(EC=2.7.7.6) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This enzyme is the central component of the basal RNA polymerase II transcription machinery. Synonymes:DNA-directed RNA polymerase III largest subunit,DNA-directed RNA polymerase II subunit RPB1.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana
• Not reactive in: Physcomitrium patens
• Immunogen: KLH-conjugated synthetic peptide derived from known higher plant polymerase II sequences, includingArabidopsis thalianaP18616,At4g35800
• Clonality: Polyclonal
• Applications: Chromatin Immuno Precipitation (ChIP), Immunoprecipitation (IP)
• Dilution: 1,2 or 5 µg (ChIP, IP)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 204 kDa
• Precautions: This antibody is not suitable for western blot in denatured conditions
• References & Citations: Crawfordet al. (2024).The Mediator kinase module enhances polymerase activity to regulate transcriptional memory after heat stress in Arabidopsis. EMBO J. 2024 Feb;43(3):437-461.Wanget al. (2020). Close arrangement of CARK3 and PMEIL affects ABA-mediated pollen sterility in Arabidopsis thaliana. Plant Cell Environ. 2020 Aug 20.doi: 10.1111/pce.13871.GodoyHerz et al. (2019). Light Regulates Plant Alternative Splicing through the Control of Transcriptional Elongation. Mol Cell. 2019 Jan 15. pii: S1097-2765(18)31035-9. doi: 10.1016/j.molcel.2018.12.005.Dolataet al. (2015). NTR1 is required for transcription elongation checkpoints at alternative exons in Arabidopsis. EMBO J. 2015 Jan 7. pii: e201489478.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.