Anti-UAGPase | UDP-GlcNAc pyrophosphorylase

• Background:

  UAGPase (UDP-GlcNAc pyrophosphorylase)is involved in the biosynthesis of UDP-glucosamine, an essential precursor for glycoprotein and glycolipid synthesis and is also used for regulatory protein modification in signaling pathways. The enzyme is localized in a cytoplasm. Alternative names: N-acetylglucosamine-1-phosphate uridylyltransferase 2, UDP-N-acetylgalactosamine diphosphorylase 2, UTP--glucose-1-phosphate uridylyltransferase 2, AGX.

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Hordeum vulgare, Nicotiana tabacum

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  His-tagged full length recombinant UAGPase ofArabidopsis thaliana, overexpressed and purified fromE.coli,UniProt:O64765,, TAIR:AT2G35020.Sequence used for immunization is conserved in both isoforms: UDP-N-acetylglucosamine diphosphorylase 1 (Q940S3) and (O64765)

• Clonality:

  Polyclonal

• Applications:

  Western blot (WB)

• Dilution:

  1 : 10 000 (WB)

• Purity:

  Serum

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 50 µl of sterile water

• Molecular Weight:

  55.76 kDa

• Precautions:

  This antibody is recognizing recombinant UAGPase at 0,25 pmol

• References & Citations:

  Fernández-SanMillán et al. (2018). Physiological Performance of Transplastomic Tobacco Plants Overexpressing Aquaporin AQP1 into Chloroplast Membranes. J Exp. Bot. ery148, https://doi.org/10.1093/jxb/ery148.KleczkowskiLA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. 2015 Volume 62 Issue 2 Pages 25-36.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4