Anti-UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker)

Anti-UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker)

• Background: UDP-glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9.  is a key enzyme of synthesis of sucrose, cellulose and other saccharides. There are two cytoplasmic isoforms of UGPase-A (which share 94 % identity on amino acid level) and one chloroplastic UGPase-B isoform inArabidopsis thalianawhich share ca. 10-11 % of identity (Kleczkowski et al. 2011).Alternative name: UTP--glucose-1-phosphate uridylyltransferase.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Hordeum vulgare, Zea mays
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: His-tagged, full lengthHordeum vulgareUGPase, overexpressed and purified fromE.coli, UniProt:Q43772.1
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 10 000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 52 kDa
• Precautions: This antibody is also recognizing recombinant UGPase, below 0,5 pmol
• References & Citations: Martín-Merchánet al. (2024). Arabidopsis AGO1 N-terminal extension acts as an essential hub for PRMT5 interaction and post-translational modifications. Nucleic Acids Res . 2024 May 20:gkae387.doi: 10.1093/nar/gkae387.KleczkowskiLA & Decker DD (2015) Sugar activation for production of nucleotide sugars as substrates for glycosyltransferases in plants. J. Appl. Glycosci. (in press).
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.