Donkey anti-Sheep IgG (H&L), DyLight® 800 conjugated, min, cross-reactivity to human, mouse, or rabbit IgG

• Background:

  Donkey anti-Sheep IgG (H&L), DyLight®800 Conjugated, min. cross-reactivity to human,mouse, or rabbit IgGis a secondary antibody conjugated toDyLight® 800, which binds to Sheep IgG (H&L) in immunological assays.DyLight® 800 has Amax = 777 nm, Emax = 794 nm. Antibodies are purified using solid phase Sheep IgG (H&L)DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

• CAS Number:

  9007-83-4

• Host:

  Donkey

• Immunogen:

  Purified Sheep IgG, whole molecule

• Clonality:

  Polyclonal

• Applications:

  immunofluorescence (IF), IImmunohistochemisty (IHC), Western blot (WB)

• Dilution:

  1 : 20-1 : 2000 for most applications

• Purity:

  Immunogen affinity purified donkey IgG.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily

• Storage Conditions:

  Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.