Donkey anti-Sheep IgG (H&L), HRP conjugated

• Background:

  Donkey anti-sheep IgG is a secondary antibody conjugated to HRP (horse radish peroxidase) which binds to all sheep immunoglobulins in immunological assays.

• HS Code:

  38221900

• Host:

  Donkey

• Reactivity:

  Sheep IgG heavy chains and light chains on all sheep immunoglobulins

• Not reactive in:

  Non-immunoglobulin sheep serum proteins based in immunoelectrophoresis

• Immunogen:

  Purified sheep IgG, whole molecule

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

• Field of Research:

  Anti-Sheep

• Dilution:

  1 : 200 -1 : 5 000 (ELISA), 1 : 200-1 : 5 000 (WB) 1 : 500 -1 : 5000 (IHC)

• Purity:

  Immunogen affinity purified donkey IgG.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily

• Additionnal Information:

  Antibody concentration is 1 mg/ml and it has been purified by antigen-specific chromatographyHRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of ProClin 150 is used as preservative.For blocking: BSA and nonfat milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

• Storage Conditions:

  Store lyophilized material at 2-8°C. For storage at -20°C after reconstitution dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

• CAS Number:

  9007-83-4