Donkey anti-Sheep IgG (H&L), DyLight® 633 conjugated, min, cross-reactivity to human, mouse, or rabbit IgG

• Background:

  Donkey anti-Sheep IgG (H&L) - DyLight® 633 Conjugated, min. cross-reactivity to human,mouse, or rabbit IgGis a secondary antibody conjugated toDyLight® 633, which binds to sheep IgG (H&L) in immunological assays.DyLight® 633 has Amax = 638 nm, Emax = 658 nm. Antibodies are are affinity purified using solid phase sheep IgG (H&L).DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

• CAS Number:

  9007-83-4

• Host:

  Donkey

• Immunogen:

  Purified sheep IgG, whole molecule

• Clonality:

  Polyclonal

• Dilution:

  1 : 20-1 : 2000 for most applications

• Purity:

  Immunogen affinity purified donkey IgG.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 1.1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily

• Storage Conditions:

  Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.