Anti-HTA9 | Probable histone H2A variant 3

Anti-HTA9 | Probable histone H2A variant 3

• Background: Variant histone H2A may replace conventional H2A in a subset of nucleosomes. Synonymes: H2A.F/Z 3
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana
• Not reactive in: Nicotiana tabacum, Zea mays
• Immunogen: KLH-conjugated synthetic peptide derived fromArabidopsis thaliana HTA9 UniProt:Q9C944, TAIR:At1g52740
• Clonality: Polyclonal
• Applications: Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP),  Western blot (WB)
• Dilution: 6 µg (ChIP), 1: 100 (IP), 1 : 1000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 14.2 kDa (Arabidopsis thaliana)
• References & Citations: Abelendaet al. (2022) High ambient temperature impacts on flowering time in Brassica napus through both H2A.Z-dependent and independent mechanisms [published online ahead of print, 2022 Dec 27]. Plant Cell Environ. 2022;10.1111/pce.14526. doi:10.1111/pce.14526Sun, Yin, Ma, et al. (2022) Feedback regulation of auxin signaling through the transcription of H2A.Z and deposition of H2A.Z to SMALL AUXIN UP RNAs in Arabidopsis. New Phytol. 2022;236(5):1721-1733. doi:10.1111/nph.18440Bieluszewskiet al. (2022) NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis. Nat Commun. 2022 Jan 12;13(1):277. doi: 10.1038/s41467-021-27882-5. PMID: 35022409; PMCID: PMC8755797.Bieluszewskiet al. (2022) NuA4 and H2A.Z control environmental responses and autotrophic growth in Arabidopsis. Nat Commun. 2022 Jan 12;13(1):277. doi: 10.1038/s41467-021-27882-5. PMID: 35022409; PMCID: PMC8755797.Kralemannet al. (2020). Removal of H2Aub1 by ubiquitin-specific proteases 12 and 13 is required for stable Polycomb-mediated gene repression in Arabidopsis. Genome Biol. 2020 Jun 16;21(1):144.doi: 10.1186/s13059-020-02062-8.Gómez-Zambranoet al. (2019). The repressive role of Arabidopsis H2A.Z in transcriptional regulation depends on AtBMI1 activity. Nat Commun. 2019 Jun 27;10(1):2828. doi: 10.1038/s41467-019-10773-1.Gómez-Zambranoet al. (2018). Arabidopsis SWC4 Binds DNA and Recruits the SWR1 Complex to Modulate Histone H2A.Z Deposition at Key Regulatory Genes. Mol Plant. 2018 Mar 29. pii: S1674-2052(18)30122-9. doi: 10.1016/j.molp.2018.03.014.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.