Anti-Histone H2B (Schizosaccharomyces pombe)

• Background:

  In the eukaryotic cells, DNA is packaged repetitively into nucleosomes by means of interactions among two molecules of four classes of histone, H2A, H2B, H3 and H4. Each of the histone proteins has an evolutionarily conserved amino-terminal ‘tail’ that protrudes from the nucleosome. This tail is the target of numerous diverse signaling pathways, resulting in the addition of many post-translational modifications. These modifications include phosphorylation, acetylation, methylation, ADP-ribosylation and mono-ubiquitination. Many important new modifications within the structured core and the carboxy-terminal tail regions of histones are also being identified. It is becoming increasingly clear that these modifications represent crucial regulatory events that govern the accessibility and function of the genome.

• Host:

  Rabbit

• Reactivity:

  Schizosaccharomyces pombe

• Immunogen:

  Synthetic peptide corresponding to N-terminalSchizosaccharomyces pombehistone H2B, SAAEKKPASKAPAGKA, UniProt:P04913

• Clonality:

  Polyclonal

• Applications:

  Chromatin immunoprecipitation (ChIP), Western blot (WB)

• Dilution:

  1 : 1000 (WB)

• Purity:

  Serum. Contains 0.05% sodium azide.

• Format:

  Liquid

• Molecular Weight:

  13.8 | 17, 24-25 kDa (unmodified and mono-ubiquinated H2B)

• Precautions:

  ChIP method for this antibody is described inMaruyamaet al. (2006).

• References & Citations:

  Maruyamaet al (2006). Histone H2B mutations in inner region affect ubiquitination, centromere function, silencing and chromosome segregation. EMBO J. 2006 Jun 7;25(11):2420-31. doi: 10.1038/sj.emboj.7601110. Epub 2006 May 11. PMID: 16688222; PMCID: PMC1478186.

• Storage Conditions:

  Store at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4