Goat anti-Rat IgG (H&L), ALP conjugated, min, reactivity to Human and mouse IgG, highly adsorbed against mouse IgG

• Background:

  Goat anti-rat IgGis a secondary antibody conjugated to ALP (Alkaline phosphatase) which binds to all donkey immunoglobulins inimmunological assays.

• Host:

  Goat

• Reactivity:

  Heavy chains on Rat IgG and with the light chains on all Rat immunoglobulins based on IEP

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Purified Rat IgG, whole molecule

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA) , Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western blot (WB)

• Dilution:

  1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

• Purity:

  Immunogen affinity purified goat IgG.

• Format:

  Liquid

• Precautions:

  This antibody is highly cross absorbed against mouse IgG, No reactivity is observed to non-immunoglobulin rat serum proteins based on immunoelectrophoresis, No reactivity is observed to human or mouse IgG based on immunoelecrophoresis,

• References & Citations:

  Liet al. (2022), The effects of Ni availability on H2 production and N2 fixation in a model unicellular diazotroph: The expression of hydrogenase and nitrogenase. Limnol Oceanogr, 67: 1566-1576. https://doi.org/10.1002/lno.12151

• Storage Conditions:

  Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

• CAS Number:

  9007-83-4