Goat anti-Rat IgG (H&L), HRP conjugated

• Background:

  Goat anti-rat IgG (H&L), HRP conjugatedis a secondary antibody which binds to rat IgGs in immunological assays. Antibodies are affinity purified using solid phase rat IgG.

• Host:

  Goat

• Reactivity:

  Heavy chains on rat IgG and with the light chains on all rat immunoglobulins

• Not reactive in:

  Non-immunoglobulin rat serum proteins

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)

• Dilution:

  The optimal working dilution should be determined by the investigator

• Purity:

  Immunogen affinity purified rat IgG.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Centrifuge to remove any particulates. Prepare fresh working dilutions daily.

• References & Citations:

  Saintenacet al. (2021) A wheat cysteine-rich receptor-like kinase confers broad-spectrum resistance against Septoria tritici blotch. Nat Commun. 2021 Jan 19;12(1):433. doi: 10.1038/s41467-020-20685-0. PMID: 33469010; PMCID: PMC7815785.

• Storage Conditions:

  Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Store liquid material at 2-8°C up to 6 months.

• CAS Number:

  9007-83-4