Goat anti-Rat IgG (H&L), F(ab)'2 fragment, DyLight® 488 conjugated, min, cross-reactivity to human, mouse lgG

• Background:

  Goat anti-Rat IgG (H&L) - F(ab)'2 fragment, DyLight488 Conjugated, min. cross-reactivity to human, mouse lgGis a secondary antibody conjugated toDyLight® 488, which binds to Rat IgG (H&L) - F(ab)'2 fragment in immunological assays.DyLight® 488 has Amax = 493 nm, Emax = 518 nm. Antibodies are purified using solid phase Rat IgG (H&L)DyLight® is a registered trade mark of Thermofisher Inc., and its subsidaries.

• CAS Number:

  9007-83-4

• Host:

  Goat

• Immunogen:

  Purified Rat IgG, whole molecule

• Clonality:

  Polyclonal

• Dilution:

  1 : 20-1 : 2000 for most applications

• Purity:

  Immunogen affinity purified goat IgG.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 0.55 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily

• Storage Conditions:

  Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0,5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.