Goat anti-Rat IgG (H&L), TRITC conjugated, min, cross-reactivity to human, mouse IgG

• Background:

  Goat anti-rat IgG (H&L), Rhodamine (TRITC-tetramethylrhodamine-5-isothiocyanate) conjugated,is a secondary antibody which binds to rat IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibodies are adsorbed against human,mouse IgG and affinity purified using solid phase rat IgG.

• CAS Number:

  9007-83-4

• Host:

  Goat

• Clonality:

  Polyclonal

• Dilution:

  1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC) (Flow cyt)

• Purity:

  Immunogen affinity purified IgG.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 1.1 ml of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily

• Storage Conditions:

  Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.