Anti-BiP | Lumenal-binding protein (goat antibody)

• Background:

  BiP2(Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER.  BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2

• Host:

  Goat

• Reactivity:

  Arabidopsis thaliana, Hordeum vulgare, Spinacia oleracea, Zea mays

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  KLH-conjugated synthetic peptide derived fromArabidopsis thalianaBiP proteins: BiP1 UniProt:Q9LKR3, TAIR:At5g28540, BiP2 UniProt:F4K007, TAIR:At5g42020, BiP3 UniProt:Q8H1B3,TAIR:At1g09080

• Clonality:

  Polyclonal

• Applications:

  Western blot (WB)

• Dilution:

  1 : 2000 (WB)

• Purity:

  Immunogen affinity purified serum in PBS pH 7.4.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 100 µl of sterile water

• Molecular Weight:

  73.5 | 80 kDa

• Precautions:

  Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Antibody has a reduced reactivity to monocots in western blot.

• References & Citations:

  Narusakaet al (2016). Leucine zipper motif in RRS1 is crucial for the regulation of Arabidopsis dual resistance protein complex RPS4/RRS1. Sci Rep. 2016 Jan 11;6:18702. doi: 10.1038/srep18702.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4