Anti-BiP | Lumenal-binding protein (chicken antibody)

• Background:

  BiP2(Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER.  BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER). Alternative name: AtBP2

• Host:

  Chicken

• Reactivity:

  Arabidopsis thaliana, Hordeum vulgare, Physcomitrium patens, Spinacia oleracea,Zea mays

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  KLH-conjugated synthetic peptide derived fromArabidopsis thalianaBiP proteins: BiP1 UniProt:Q9LKR3, TAIR:At5g28540, BiP2 UniProt:F4K007, TAIR:At5g42020, BiP3 UniProt:Q8H1B3,TAIR:At1g09080

• Clonality:

  Polyclonal

• Applications:

  Immunofluorescence (IF), Western blot (WB)

• Dilution:

  1 : 50-1 : 1000 (IF), 1 : 2000 (WB)

• Purity:

  Immunogen affinity purified total IgY. in PBS pH 7.4.

• Format:

  Liquid

• Molecular Weight:

  73,5 | 80 kDa

• Precautions:

  Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel

• References & Citations:

  Bennettet al. (2014). Plasma Membrane-Targeted PIN Proteins Drive Shoot Development in a Moss. Curr Biol. 2014 Dec 1;24(23):2776-85. doi: 10.1016/j.cub.2014.09.054. Epub 2014 Nov 13.

• Storage Conditions:

  Store at 4°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4