Anti-HydA2 | Iron-hydrogenase HydA2

• Background:

  Iron-hydrogenase HydA2 is catalyzing reversible oxidation of molecular hydrogen. Chlamydomonas the protein is present in low levels of 1 µg/liter of culture. Synonymnes: HYD2

• HS Code:

  38221900

• Gene ID:

  5720168

• UniProt:

  Q8VZZ0

• Host:

  Rabbit

• Reactivity:

  Chlamydomonas reinhardtii, recombinant HydA2 expressed in E.coli

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  Recombinant, full-length Chlamydomonas reinhardtii HydA-2 UniProt: Q8VZZ0

• Clonality:

  Polyclonal

• Applications:

  Western blot (WB)

• Field of Research:

  Fermentation

• Dilution:

  1 : 5000 (WB)

• Purity:

  Serum

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 100 µl of sterile water

• Molecular Weight:

  53.7 | 47.3 kDa (after a signal peptide is cleaved)

• Precautions:

  HydA2 (505aa) has a calculated MW of 53.7 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP can only be estimated, since the cleavage site is known only from in silico analysis. It has a calculated MW of 47.3 kDa and should run in the gel also according to its size.HydA1 (497aa) has a calculated MW of 53.1 kDa, but this is including the signal peptide, which gets cleaved off. The protein without TP has a calculated MW of 47.5 kDa and runs according to its size at about 48 kDa

• Additionnal Information:

  In Chlamydomonas HydA is present in low levels of 1 µg/liter of culture. Therefore, an induction of cells by anaerobic adaptation or sulfur depravation (10 x higher amount than with anaerobic adaptation) is necessary for successful detection using this antibody. Methods of HydA induction are described in Hemschemeier et al. 2009.To detect HydA in Chlamydomonas extracts amount loaded per well corresponds to 2 µg of chlorophyll for sulfur deprived cells, where relatively much HydA1 is synthesized or corresponds to 2-4 µg of artificially anaerobic induced cultures, where the HydA1 protein level is lower.This antibody is recognizing 1 ng of recombinant HydA protein.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• Prediction Reactivity:

  Ostreococcus sp.Species of your interest not listed? Contact us

• CAS Number:

  9007-83-4