Anti-V-ATPase | Epsilon subunit of tonoplast H+ATPase (affinty purified, goat antibody)

• Background:

  Plant vacuoleV-ATPaseis responsible for energization of transport of ions and metabolites, and acts as well 'house-keeping' and as a stress response enzyme. V-ATPase is a multi-subunit enzyme composed of a membrane sector and a cytosolic catalytic sector. It is related to the FoF1 ATP synthase. Alternative protein names: Vacuolar proton pump subunit E, Protein EMBRYO DEFECTIVE 2448

• Host:

  Goat

• Reactivity:

  Arabidopsis thaliana, Avena strigosa, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Solanum lycopersicum

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  KLH-conjugated synthetic peptide chosen from subunit E of plant V-ATPase includingArabidopsis thalianaAt4g11150. Peptide is conserved in vacuolar H+-ATPase subunit E, isoform 1 to 3 (VHA-E1).

• Clonality:

  Polyclonal

• Applications:

  Immunofluorescence (IF), Western blot (WB)

• Dilution:

  1: 600 (IF), 1 : 1000-1 : 3000 (WB)

• Purity:

  Immunogen affinity purified serum in PBS pH 7.4.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 100 µl of sterile water

• Molecular Weight:

  26 | 31 kDa (Arabidopsis thaliana)

• Precautions:

  V-ATPase is very sensitive for the redox of the SDS buffer. We recommend using at least 50-100 mM DTT freshly prepared before handling the sample.2 hours incubation with primary antibody is recommended over over night incubation which can contribute to increased background.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4