Anti-PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5 | Aquaporins

• Background:

  PIPsproteins are aquaporins which facilitate the transport of water and small neutral molecules across cell membrane. PIP1;2 is also designated as PIP1B.

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Nicotiana tabacum, Phoenix dactilifera, Raphanus sativus, Zea mays

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  KLH-conjugated synthetic peptide derived from N terminus ofRaphanus sativusPAQ1O80368. Chosen peptide is conserved in PIP1;1, PIP1;2, PIP1;3 N-terminus ofRaphanus sativusand in  all 5 isoforms ofArabidopsis thalianacoded by:AT3G61430.1(PIP1;1),AT2G45960.3(PIP1;2),AT1G01620.1(PIP1;3),AT4G00430.1(PIP1;4),AT4G23400.1(PIP1;5)

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Western blot (WB)

• Dilution:

  1 : 8000 (ELISA), 1 : 1000 (WB)

• Purity:

  Serum

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 100 µl of sterile water

• Molecular Weight:

  30.66 | 28 kDa (Raphanus sativus)

• Precautions:

  Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.Diluted antibody solution can be used 2 to 3 times within one month if it  contains 0.1 % sodium azide as preservative and is stored at -20ºC to -80ºC.Antibody reacts also with PIP2 isoforms inZea mays.

• References & Citations:

  Patankaret al. (2019). Functional Characterization of Date Palm Aquaporin Gene PdPIP1;2 Confers Drought and Salinity Tolerance to Yeast and Arabidopsis. Genes (Basel). 2019 May 22;10(5). pii: E390. doi: 10.3390/genes10050390.Fernández-SanMillán et al. (2018). Physiological Performance of Transplastomic Tobacco Plants Overexpressing Aquaporin AQP1 into Chloroplast Membranes. J Exp. Bot. ery148, https://doi.org/10.1093/jxb/ery148.Pengellyet al. (2014). Transplastomic integration of a cyanobacterial bicarbonate transporter into tobacco chloroplasts. J Exp. Botany, doi:10.1093/jxb/eru156.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. For long time storage over 1 year please dilute the antibody solution 10 fold with 10 mM Tris-buffered saline containing 10% glycerol. Glycerol may suppress denaturation of the protein.

• CAS Number:

  9007-83-4