Anti-PIP (PIP1;1, PIP1;2, PIP1;3, PIP1;4, PIP1;5) | Aquaporins

• Background:

  PIPsproteins are aquaporins which facilitate the transport of water and small neutral molecules across the cell membrane. Alternative names: PIP1-1, PIP1-2, PIP1-3, plasma membrane intrinsic protein 1a, 1b, 1c, PIP1a, PIP1b, PIP1c, plasma membrane aquaporin-1, transmembrane protein A, TMP-A, AthH2, transmembrane protein B, TMP-B.

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana, Brassica sp., Jatropha curcasL. cv. Biji Jarak ,Mesembryantheum crystallinum,Populus nigra, Populus trichocarpa,Raphanus sativus, Setaria viridis, Thellungiella salsuginea

• Not reactive in:

  Fragariasp.,Spinacia oleracea

• Immunogen:

  KLH-conjugated synthetic peptide conserved inArabidopsis thaliana:PIP1;1 UniProt:P61837,At3g61430PIP1;2 UniProt:Q06611,TAIR:At2g45960PIP1;3 UniProt:Q08733,TAIR:At1g01620, PIP1;4 UniProt:Q39196,TAIR:At4g00430, PIP1;5 UniProt:Q8LAA6TAIR:At4g23400

• Clonality:

  Polyclonal

• Applications:

  Immunofluorescence (IF), Western blot (WB)

• Dilution:

  1: 150 (IF), 1 : 1000 (WB)

• Purity:

  Immunogen affinity purified serum in PBS pH 7.4.

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 50 µl of sterile water

• Molecular Weight:

  30.68 | 28 kDa

• Precautions:

  Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.

• References & Citations:

  Takáčet al. (2024). Actin cytoskeleton and plasma membrane aquaporins are involved in different drought response of Arabidopsis rhd2 and der1 root hair mutants. Plant Physiology and Biochemistry Available online 19 September 2024, 109137.Chenet al. (2022) Elucidating the role of SWEET13 in phloem loading of the C4 grass Setaria viridis. Plant J. 2022 Feb;109(3):615-632. doi: 10.1111/tpj.15581. Epub 2021 Dec 12. PMID: 34780111.Janget al. (2013). Twoaquaporins of Jatropha are regulated differentially during drought stress and subsequent recovery. J Plant Physiol. March 25.Lopezet al. (2013). Aquaporins And Leaf Hydraulics, Poplar Sheds New Light. Plant Cell Physiol. Sep 20.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4