Anti-SUMO1 | Small ubiquitin-like modifier protein 1

Anti-SUMO1 | Small ubiquitin-like modifier protein 1

• Background: SUMO1-SmallUbiquitin-likeModifier ubiquitin like protein binds in reversible way to various protein targets and plays a role as a signaling regulator.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Oryza sativa, Solanum tuberosum
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Recombinant proSUMO1 fromArabidopsis thalianaQ547B9,At4g26840with a his tag
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 1000-1 : 5000 (WB)
• Purity: Total IgG. Protein G purified in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 50 µl of sterile water
• Molecular Weight: 10.97 | 12 kDa
• Precautions: Antibodies will also detect SUMO2 protein.Suggested extraction buffer: 100 mM Tris-HCl, pH 8.0, 0.1% [w/v] SDS, 0.5% [w/v] sodium deoxycholate, 1% [v/v] glycerol, 50 mM sodium metabisulfite, 20 mM N-ethylmaleimide (NEM) and protease inhibitor cocktail (Roche) (Orosaet al. 2018). This buffer will help to stabilize the conjugates and will help to detect any increase or decrease in conjugate accumulation using the antibodies.
• References & Citations: Xiaoet al. (2024). Small ubiquitin-like modifier protease gene TaDSU enhances salt tolerance of wheat. New Phytologist, October 2024.Szadeczky-Kardosset al. (2022) Elongation factor TFIIS is essential for heat stress adaptation in plants. Nucleic Acids Res. 2022 Feb 28;50(4):1927-1950. doi: 10.1093/nar/gkac020. PMID: 35100405; PMCID: PMC8886746.Colignonet al. (2019). Dual coordination of the SUMOylation and phosphorylation pathways during the response to heat stress in Solanum tuberosum. Environmental and Experimental Botany Volume 162, June 2019, Pages 192-200.Rosaet al. (2018). Insights into the transcriptional and post-transcriptional regulation of the rice SUMOylation machinery and into the role of two rice SUMO proteases. BMC Plant Biol. 2018 Dec 12;18(1):349. doi: 10.1186/s12870-018-1547-3.Guoet al. (2017). Sumoylation stabilizes RACK1B and enhance its interaction with RAP2.6 in the abscisic acid response. Sci Rep. 2017 Mar 8;7:44090. doi: 10.1038/srep44090.Tomanovet al. (2014). Arabidopsis PIAL1 and 2 Promote SUMO Chain Formation as E4-Type SUMO Ligases and Are Involved in Stress Responses and Sulfur Metabolism. Plant Cell. 2014 Nov;26(11):4547-60. doi: 10.1105/tpc.114.131300.Liuet al. (2014). SUMO E3 ligase AtMMS21 is required for normal meiosis and gametophyte development in Arabidopsis. BMC Plant Biol. 2014 Jun 3;14:153. doi: 10.1186/1471-2229-14-153.Konget al. (2014). Quantitative proteomics analysis reveals that the nuclear cap-binding complex proteins Arabidopsis CBP20 and CBP80 modulate the salt stress response. J Proteome Res. 2014 Apr 1.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.