Anti-GPX | Glutathione peroxidase (chloroplastic)

• Background:

  GPXbelongs to the group of enzymes that protect cells against oxidative damage generated by reactive oxygen species (ROS). Glutathione peroxidase catalyzes the reduction of H2O2, organic hydroperoxides, and lipid peroxides in the presence of glutathione, the hydrogen donor. Activity of the enzyme can be increased under environmental stress. Immunogen: Peptide target chosen from C-terminal of two chloroplastic isoforms of Arabidopsis thaliana GPX.

• Host:

  Rabbit

• Reactivity:

  Arabidopsis thaliana

• Not reactive in:

  No confirmed exceptions from predicted reactivity are currently known

• Immunogen:

  thioredoxin fusion of 58 amino acid region between Ser at position 175 and the C-terminal Ala ofArabidopsis thalianaGPX7 accessionAt4g31870has been used for antibody production

• Clonality:

  Polyclonal

• Applications:

  ELISA (ELISA), Western blot (WB)

• Dilution:

  1 : 1, 1 : 2, 1 :4, 1 :8, 1 : 16 (ELISA), 1 : 5000 (WB)

• Purity:

  Serum

• Format:

  Lyophilized

• Reconstitution:

  For reconstitution add 100 µl of sterile water

• Molecular Weight:

  25 | 20 kDa

• Precautions:

  This antibody is recognizingArabidopsis thalianaGPX (two chloroplastic isoforms). Antibody has also a weak cross-reactivity to cytosolic forms, therefore work on isolated chloroplasts is recommended.

• References & Citations:

  Lepistöet al. (2013). Deletion of chloroplast NADPH-dependent thioredoxin reductase results in inability to regulate starch synthesis and causes stunted growth under short-day photoperiods. J Exp Bot. July 23.Juszczaket al. (2012). Natural genetic variation in the expression regulation of the chloroplast antioxidant system among Arabidopsis thaliana accessions. Physiol. Plant.

• Storage Conditions:

  Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

• CAS Number:

  9007-83-4