Anti-PEPCK | PEP carboxykinase

Anti-PEPCK | PEP carboxykinase

• Background: Phosphoenolpyruvate carboxykinase (PEPCK, PEP carboxykinase) is an enzyme that catalyses the conversion of oxaloacetate and ATP to phosphoenelpyruvate, carbon dioxide and ADP. PEPCK is encoded by two genes in plants: pck1 and pck2.
• Host: Rabbit
• Reactivity: Ananas comosus, Miscantus giganteus, Mouse, Nannochloropsis oceanica, Oryza sativa, Panicum maximum,, Panicum virgatum, Phaseolus vulgaris, Saccharumspp. hybrid clone C91-301,Setaria viridis, Spartina alterniflora, Spartina patens, Zea mays
• Not reactive in: Arabidopsis thaliana, Pinus yunnanensis
• Immunogen: KLH-conjugated synthetic peptide well conserved in both PEPCK1 and 2 sequences from different plant species includingZea maysQ9SLZ0
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 1000 (WB)
• Purity: Serum
• Format: Lyophilized
• Reconstitution: For reconstitution add 200 µl of sterile water
• Molecular Weight: 73 | 78 kDa
• Precautions: Due to the MW of this protein we suggest to use a gradient gel for protein separation and a longer transfer time. Higher protein load 10-20 µg is adviced when working with this antibody.Antibody can be also used following 2D gel electrophoresis.This product can be sold containing ProClin if requested.
• References & Citations: Weiet al. (2019). Transcriptomic and proteomic responses to very low ­CO 2 suggest multiple carbon concentrating mechanisms in Nannochloropsis oceanica. Biotechnol Biofuels 12: 168.Shenet al. (2016). The existence of C4-bundle-sheath-like photosynthesis in the mid-vein of C3 rice. Rice (N Y). 2016 Dec;9(1):20. doi: 10.1186/s12284-016-0094-5. Epub 2016 May 10.Aragónet al. (2013). The physiology of ex vitro pineapple (Ananas comosus L. Merr. var MD-2) as CAM or C3 is regulated by the environmental conditions: proteomic and transcriptomic profiles. Plant Cell Rep. Aug 20. (Ananas comosus, western blot detection following 2D gel electrophoresis)
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.