Anti-PR-3 / CHN | Class I chitinase

Anti-PR-3 / CHN | Class I chitinase

• Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known.  Glucanase I  (PR-2) and chitinase I (PR-3) contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Agostis stoloniferacv. ‘Penncross’,Capsicum annuum, Nicotiana tabacum, Picea abies, Solanum esculentum, Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: Purified tobacco class I chitinase. The preparation used is a mixture of two class I isoforms (Shinshi et al., 1990; van Buuren et al., 1992): 1) Chitinase A (CHN A)P08252encoded by gene chn48 derived from theN. tomentosiformisancestor of tobacco. 2) Chitinase B (CHN B)P24091encoded by gene chn50 derived from theN. sylvestrisancestor of tobacco.
• Clonality: Polyclonal
• Applications: Co-Immunoprecipitation (IP) (Co-IP), Immunolocalization (IL), Western blot (WB)
• Dilution: 8 µg/mL (WB)
• Purity: Total IgG. Protein G purified in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 100 µl of sterile water
• Molecular Weight: 35, 34 | 32 and 34 kDa
• Precautions: Important note: For blocking 5% skim milk in PBS without Ca++ should be used. This antibody is purified by affinity chromarography on Portein G.
• References & Citations: Mansillaet al. (2020).- Characterization of functionalized bentonite as nanocarrier of salicylic acid with protective action against Pseudomonas syringae in tomato plants. Eur J Plant Pathol 158, 211?222 (2020). https://doi.org/10.1007/s10658-020-02067-wColmanet al. (2019). Chitosan microparticles improve tomato seedling biomass and modulate hormonal, redox and defense pathways. Plant Physiology and Biochemistry Volume 143, October 2019, Pages 203-211.Kumariet al. (2017), Overexpression of a Plasma Membrane Bound Na+/H+ Antiporter-Like Protein (SbNHXLP) Confers Salt Tolerance and Improves Fruit Yield in Tomato by Maintaining Ion Homeostasis. Front Plant Sci. 2017 Jan 6;7:2027. doi: 10.3389/fpls.2016.02027.Jespersenet al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ?Penncross?)Koet al. (2016). Constitutive expression of a fungus-inducible carboxylesterase improves disease resistance in transgenic pepper plants. Planta. 2016 Aug; 244(2):379-92. doi: 10.1007/s00425-016-2514-6. Epub 2016 Apr 13.AnilKumar et al. (2016). Beyond just being foot soldiers – osmotin like protein (OLP) and chitinase (Chi11) genes act as sentinels to confront salt, drought, and fungal stress tolerance in tomato. Environmental and Experimental Botany 132 (2016) 53–65.Wuet al. (2016). Laminarin modulates the chloroplast antioxidant system to enhance abiotic stress tolerance partially through the regulation of the defensin-like gene expression. Plant Science, Volume 247, June 2016, Pages 83–92.Falcioniet al. (2013). Effect of salicylic acid treatment on tomato plant physiology and tolerance to potato virus X infection. Eur J Plant Pathol DOI 10.1007/s10658-013-0333-1.Mungeret al. (2012). Beneficial 'unintended effects' of cereal cystatin in transgenic lines of potato, Solanum tuberosum. BMC Plant Biology, 12:198.Sticheret al. (1993). Posttranslational processing of a new class of hydroxyproline-containing proteins: Prolyl hydroxylation and C-terminal cleavage of tobacco (Nicotiana tabacum) vacuolar chitinase. Plant Physiol. 101, 1239-1247.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.