Anti-SPS | Sucrose phosphate synthase, global

Anti-SPS | Sucrose phosphate synthase, global

• Background: SPS(sucrose phosphate synthase, EC 2.4.1.14) is the key enzyme of carbon flux into sucrose fixation in plants. It catalyzes the synthesis of sucrose-phosphate from UDP-glucose and fructose-6-phosphate predominantly in the cytosol of sucrose-source leaf tissue.
• CAS Number: 9007-83-4
• Host: Rabbit
• Reactivity: Arabidopsis thaliana, Brassica napus, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum penelli, Miscanthus x giganteus, Pinus strobus, Solanum tuberosum, Triticum aestivum, Pinus strobus, Zea mays
• Not reactive in: No confirmed exceptions from predicted reactivity are currently known
• Immunogen: KLH-conjugated synthetic peptide derived from conserved region within plant SPS protein sequences, includingArabidopsis thalianaisoforms 1FQ94BT0, 2F, 3F and 4F.Oryza sativaQ67WN8,Solanum tuberosumQ43845
• Clonality: Polyclonal
• Applications: Western blot (WB)
• Dilution: 1 : 1000-1 : 5000 (WB)
• Purity: Immunogen affinity purified serum in PBS pH 7.4.
• Format: Lyophilized
• Reconstitution: For reconstitution add 25 µl of sterile water
• Molecular Weight: 120 | 120-130 kDa (fragments of 30-90 kDa may be detected)
• Precautions: Peptide used to elicit anti-SPS antibodies is perfectly conserved in all isoforms of SPS in plants
• References & Citations: Bilska-Koset al. (2020). Sucrose phosphate synthase (SPS), sucrose synthase (SUS) and their products in the leaves of Miscanthus× giganteus and Zea mays at low temperature. Planta . 2020 Jul 16;252(2):23. doi: 10.1007/s00425-020-03421-2.Chenet al. (2018). TIC236 links the outer and inner membrane translocons of the chloroplast. Nature. 2018 Dec;564(7734):125-129. doi: 10.1038/s41586-018-0713-y.Zhanget al. (2014). Heterologous expression of AtPAP2 in transgenic potato influences carbon metabolism and tuber development. FEBS Lett. 2014 Aug 27. pii: S0014-5793(14)00621-8. doi: 10.1016/j.febslet.2014.08.019.
• Storage Conditions: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.