SARS-CoV-2 (COVID-19) S1 protein, Fc Tag

• Background:

  It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

• Description:

  SARS-CoV-2 S1 protein, Fc Tag (S1N-C5255) is expressed from human 293 cells (HEK293) . It contains AA Val 16 - Arg 685 (Accession # QHD43416.1) .

• Specifications:

  This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 101.5 kDa. The protein migrates as 130-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  QHD43416.1

• Expression Region:

  Val 16 - Arg 685

• Host:

  HEK293

• Target:

  Spike S1

• Conjugation:

  Unconjugated

• Tag:

  C-Fc

• Source:

  SARS-CoV-2

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate,can bind SARS-CoV-2 S1 protein, Fc Tag (Cat. No. S1N-C5255) with a linear range of 0.4-6 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  Tris, Glycine and NaCl, pH7.5

• Additives:

  Trehalose

• Molecular Weight:

  101.5 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  500ug*2

• Species:

  SARS-CoV-2