SARS-CoV-2 (COVID-19) S1 protein, His Tag

• Background:

  It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

• Description:

  SARS-CoV-2 S1 protein, His Tag (S1N-C52H2) is expressed from human 293 cells (HEK293) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 76.9 kDa. The protein migrates as 110-120 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  QHD43416.1

• Expression Region:

  Val 16 - Arg 685

• Host:

  HEK293

• Target:

  Spike S1

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis

• Source:

  SARS-CoV-2

• Stability:

  ● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 12 months under sterile conditions.

• Endotoxin:

  1.0 EU per μg

• Purity:

  95%

• Bioactivity:

  Immobilized SARS-CoV-2 S1 protein, His Tag (Cat. No. S1N-C52H2) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.2-6 ng/mL (QC tested).

• Format:

  Liquid

• Buffer:

  10 mM PB, 50 mM NaCl, pH7.4

• Molecular Weight:

  76.9 kDa

• Shipping Conditions:

  Dry ice

• Storage Conditions:

  -70°C

• Package Size:

  1mg*1

• Species:

  SARS-CoV-2