Human IgG1 Fc (C103S, D239E, L241E) Protein, Tag Free (MALS verified)

Human IgG1 Fc (C103S, D239E, L241E) Protein, Tag Free (MALS verified)

• Background: Crystallizable fragments composed of the carboxy-terminal halves of both IMMUNOGLOBULIN HEAVY CHAINS linked to each other by disulfide bonds. Fc fragments contain the carboxy-terminal parts of the heavy chain constant regions that are responsible for the effector functions of an immunoglobulin (COMPLEMENT fixation, binding to the cell membrane via FC RECEPTORS, and placental transport). IgG1 Fc was reported has a novel role as a potential anti-inflammatory drug for treatment of human autoimmune diseases.
• Description: Human IgG1 Fc (C103S, D239E, L241E), Tag Free (IG1-H52H1) is expressed from human 293 cells (HEK293). It contains AA Pro 100 - Lys 330 (Accession # P01857-1).
• Specifications: This protein carries no "tag". The protein has a calculated MW of 26 kDa. The protein migrates as 31-33 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Pro 100 - Lys 330
• Host: HEK 293
• Target: IgG1 Fc
• Conjugation: Unconjugated
• Tag: Native
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Human IgG1 Fc (C103S, D239E, L241E), Tag Free (Cat. No. IG1-H52H1) at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag (Cat. No. FCA-H82E8) with a linear range of 0.3-2 ng/mL (QC tested).
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 26 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C