PDHA1 Polyclonal Antibody

• Background:

  The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO (2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) . The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

• Description:

  This is a PDHA1 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  PDHA1, PDHA, PDHAD, PDHCE1A, PHE1A

• Gene ID:

  5160

• Swiss Prot:

  P08559

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human PDHA1 (NP_000275.1) .

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC, IF

• Purification Method:

  Affinity purification

• Assay Type:

  Antibody

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:2000 IHC 1:50-1:200 IF 1:50-1:200

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  40 kDa/43 kDa/44 kDa/47 kDa

• Observed Molecular Weight:

  45 kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG