PDHK1 (Phospho-Thr338) Antibody

• Background:

  Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress.

• Description:

  This is a PDHK1 (Phospho-Thr338) Phospho-Specific Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  PDK1; PDH kinase 1

• Gene ID:

  5163

• Swiss Prot:

  Q15118

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Peptide sequence around phosphorylation site of threonine 338 (Y-S-T (p) -A-P) derived from Human PDHK1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Phospho-Specific Antibody

• Applications:

  WB

• Purification Method:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide.

• Assay Type:

  Antibody

• Concentration:

  1.0 mg/mL

• Dilution:

  WB 1:500-1:1000

• Buffer:

  Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  49kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG