MDM2 Polyclonal Antibody

• Background :

  This gene encodes a nuclear-localized E3 ubiquitin ligase. The encoded protein can promote tumor formation by targeting tumor suppressor proteins, such as p53, for proteasomal degradation. This gene is itself transcriptionally-regulated by p53. Overexpression or amplification of this locus is detected in a variety of different cancers. There is a pseudogene for this gene on chromosome 2. Alternative splicing results in a multitude of transcript variants, many of which may be expressed only in tumor cells.

• Description :

  This is a MDM2 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms :

  ACTFS, Double minute 2 protein, E3 ubiquitin-protein ligase Mdm2, Hdm 2, Hdm2, HDMX, MDM 2, MDM2, MDM2 oncogene E3 ubiquitin protein ligase, Mdm2 p53 E3 ubiquitin protein ligase homolog, Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding

• Swiss Prot :

  Q00987

• Reactivity :

  Human, Mouse, Monkey

• Immunogen :

  Synthesized peptide derived from human MDM2 around the non-phosphorylation site of Ser166.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Polyclonal Antibody

• Applications :

  WB, IHC-p, IF, ELISA

• Purification Method :

  Affinity purification

• Assay Type :

  Antibody

• Concentration :

  1 mg/mL

• Dilution :

  WB 1:500-1:2000, IHC 1:100-1:300, IF 1:200-1:1000, ELISA 1:5000

• Buffer :

  PBS with 0.02% sodium azide, 0.5% protective protein and 50% glycerol, pH7.4

• Storage Conditions :

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight :

  55 kDa

• Observed Molecular Weight :

  90 kDa

• Host or Source :

  Rabbit

• Isotype :

  IgG