Vimentin Polyclonal Antibody

• Background:

  Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein,55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level.

• Description:

  This is a Vimentin Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM, VIME, Vimentin

• Swiss Prot:

  P08670, P20152, P31000

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  KLH conjugated Synthetic peptide corresponding to Mouse vimentin

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC

• Purification Method:

  Affinity purification

• Assay Type:

  Antibody

• Concentration:

  1.1 mg/mL

• Dilution:

  WB 1:500-1:2000, IHC 1:200-1:800

• Buffer:

  PBS with 0.02% sodium azide, 1% BSA and 50% glycerol, pH7.4

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  57 kDa

• Observed Molecular Weight:

  57 kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG