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Vimentin Polyclonal Antibody

CAT: 0749-ABP52699-01Size: 30 µLDry Ice: NoHazardous: No
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CAT#:0749-ABP52699-01Size:30 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
VIM encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. Vimentin encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL) -derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
Description
Rabbit Anti-Vimentin Polyclonal Antibody
CAS Number
9007-83-4
Product Name Alternative
VIM; Vimentin
Gene ID
7431
Accession Number
P08670
Reactivity
Human, Mouse, Rat
Immunogen
Synthesized peptide derived from human Vimentin around the non-phosphorylation site of S83
Clonality
Polyclonal
Tag
Vimentin
Applications
WB, IHC, IF, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
50-57kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
Vimentin Polyclonal Antibody detects endogenous levels of Vimentin protein.
Observed Molecular Weight
50-57
Formulation
Liquid solution
Buffer pH
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.
Other Product Names
VIM; Vimentin
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/P08670
Isotype
Rabbit IgG

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