DLAT Polyclonal Antibody

• Background:

  This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC) . PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC) . In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.

• Description:

  This is a DLAT Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.

• Synonyms:

  DLAT, DLTA, PDC-E2, PDCE2

• Gene ID:

  1737

• Swiss Prot:

  P10515

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant fusion protein of human DLAT (NP_001922.2) .

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Applications:

  WB, IHC, IF

• Purification Method:

  Affinity purification

• Assay Type:

  Antibody

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:500-1:2000 IHC 1:50-1:100 IF 1:50-1:200

• Buffer:

  PBS with 0.02% sodium azide, 50% glycerol, pH7.3

• Storage Conditions:

  Store at -20°C. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  68 kDa

• Observed Molecular Weight:

  69 kDa

• Host or Source:

  Rabbit

• Isotype:

  IgG