Anti-Human CD8 Cd8a Monoclonal Antibody mFluor540 Conjugated, Flow Validated

• Description:

  Boster Bio Anti-Human CD8 Cd8a Monoclonal Antibody mFluor540 Conjugated, Flow Validated (Catalog# FC02236-mFluor540) . Tested in Flow Cytometry application (s) . This antibody reacts with Human.

• Synonyms:

  T-cell surface glycoprotein CD8 alpha chain; CD8 antigen 32 kDa chain; OX-8 membrane antigen; CD8a; Cd8a

• Gene Name:

  Cd8a

• UniProt:

  P01732

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human peripheral blood T lymphocytes

• Clonality:

  Monoclonal

• Clone:

  Clone: Clone: OKT8

• Conjugation:

  MFluor540

• Applications:

  Flow Cytometry

• Field of Research:

  Adaptive Immunity, Immunology, T Cells

• Purification:

  Affinity column chromatography

• Concentration:

  0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Dilution:

  User needs to optimize the dilution ratio for this antibody.

• Form:

  Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA

• Function:

  Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs) . In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs) . This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK- cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells. .

• Molecular Weight:

  26196 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 2-8°C. Avoid repeated freeze-thaw cycles.

• Fragment:

  IgG2a, k

• Other Gene Names:

  T-cell surface glycoprotein CD8 alpha chain

• Subcellular Location:

  Cell membrane; Single-pass type I membrane protein. CD8A localizes to lipid rafts only when associated with its partner CD8B.