Eotaxin Mouse Recombinant Protein (CCL11)

• Description:

  Eotaxin Mouse Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain containing 74 amino acids and having a molecular mass of 8403.2 Dalton. The CCL11 is purified by proprietary chromatographic techniques.

• Synonyms:

  Small inducible cytokine A11; CCL11; Eosinophil chemotactic protein; chemokine (C-C motif) ligand 11; SCYA11

• Gene Name:

  CCL11

• UniProt:

  P48298

• Reactivity:

  Mouse

• Tissue Specificity:

  Expressed constitutively in the thymus. Expression inducible in the lung (type I alveolar epithelial cells), intestine, heart, spleen, kidney.

• Source:

  Escherichia coli

• Sequence:

  [object Object]

• Purification:

  Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

• Bioactivity:

  The Biological activity was determined by measuring the dose dependent phosphorylation of ERK1 and ERK2 in CCR3 transfected 293 cells. Significant ERK phosphorylation is observed with greater than 100 ng/ml (corresponding to a Specific Activity of 10,000IU/mg) of recombinant mouse eotaxin.

• Form:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  It is recommended to reconstitute the lyophilized Eotaxin in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Function:

  In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils.

• Shipping Conditions:

  Available

• Storage Conditions:

  Lyophilized Eotaxin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CCL11 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

• Other Gene Names:

  Eotaxin

• Subcellular Location:

  Secreted.