Mouse DHFR Recombinant Protein

• Description:

  Mouse DHFR Recombinant Protein expressed in E. coli with His-tag. Sequence domain: 1-187aa. Application (s) : Enzyme Activity, SDS-PAGE. Endotoxin: < 1 EU per 1ug of protein (determined by LAL method) .

• Synonyms:

  Dihydrofolate reductase, EC 1.5.1.3

• Gene Name:

  Dhfr

• UniProt:

  P00375

• Tag:

  His-Tag

• Source:

  E. coli

• Sequence:

  [object Object]

• Applications:

  Enzyme Activity, SDS-PAGE

• Field of Research:

  Redox Proteins

• Purification:

  > 95% by SDS-PAGE

• Concentration:

  1mg/ml (determined by Bradford assay)

• Bioactivity:

  Specific activity is > 2000 pmol/min/ug, and is defined as the amount of enzyme that converts 1.0 pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25°C.

• Form:

  Liquid

• Molecular Weight:

  23.8 kDa (207aa) confirmed by MALDI-TOF

• Shipping Conditions:

  Available

• Storage Conditions:

  Can be stored at 2°C to 8°C for 1 week. For long-term storage, aliquot and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. (Ships with gel ice.)