GDNF Glial-Derived Neurotrophic Factor Human Recombinant Protein

• Description:

  Glial derived Neurotrophic Factor Human Recombinant produced in E. coli is a non-glycosylated disulfide-linked homodimer containing 2 x 135 amino acids and having a total molecular mass of approximately 30kDa. GDNF is purified by proprietary chromatographic techniques.

• Synonyms:

  ATF1; ATF2; HFB1-GDNF; GDNF

• Gene Name:

  GDNF

• UniProt:

  P39905

• Reactivity:

  Human

• Tissue Specificity:

  In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change.

• Source:

  Escherichia coli

• Sequence:

  [object Object]

• Purification:

  Greater than 95.0% as determined by (a) Analysis by RP-HPLC and (b) Analysis by SDS-PAGE.

• Bioactivity:

  507

• Form:

  Sterile Filtered White lyophilized (freeze-dried) powder.

• Reconstitution:

  It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

• Function:

  Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

• Shipping Conditions:

  Available

• Storage Conditions:

  Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GDNF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

• Other Gene Names:

  Glial cell line-derived neurotrophic factor

• Subcellular Location:

  Secreted