Rat DKK-1 PicoKine® Quick ELISA Kit, Carrier-free

• Background:

  Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

• :

  heparin or EDTA

• Description:

  Rat DKK-1 PicoKine® Quick ELISA Kit (120 minutes, 96 Tests). Quantitate Rat Dkk1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	91	93	93	92	92	0.82	0.8%
  Sample 2	361	310	365	327	340	23.09	6.7%
  Sample 3	1121	1338	1162	1204	1206	81.53	6.7%

  *number of samples for each test n=16.

• Synonyms:

  Dickkopf homolog 1 (Xenopus laevis) (Predicted) ;Dickkopf-like protein 1 ;Protein Dkk1 ;Dkk1 ;DKK1 , Dkk1_predicted , LOC100912050 ;rCG_48380 ;

• Gene Name:

  dickkopf WNT signaling pathway inhibitor 1

• Gene ID:

  293897

• UniProt:

  D3Z9J1

• Reactivity:

  Rat

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: S23-H270

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  46.9 pg/ml - 3,000 pg/ml

• Sensitivity:

  <15 pg/ml

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

• Calculated Molecular Weight:

  29149 MW

• Specificity:

  Natural and recombinant rat DKK1

• Protein Name:

  Dickkopf homolog 1