Mouse DKK-1 ELISA Kit PicoKine®

Mouse DKK-1 ELISA Kit PicoKine®

• Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
• Description: Mouse DKK-1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Dkk1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Dickkopf-related protein 1;Dickkopf-1;Dkk-1;mDkk-1;Dkk1;
• CAS Number: 7732-18-5
• Gene Name: Dickkopf-related protein 1
• Gene ID: 13380
• UniProt: O54908
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: CHO; Immunogen sequence: S30-H272
• Applications: ELISA
• Sensitivity: <15 pg/ml
• Function: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. .
• : heparin or EDTA
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Detection Range: 46.9 pg/ml - 3,000 pg/ml
• Storage Conditions: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 29298 MW
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	97	97	101	103	99	2.59	2.6%
  Sample 2	425	361	363	388	384	25.82	6.7%
  Sample 3	1418	1320	1228	1302	1317	67.74	5.1%

  *number of samples for each test n=16.
• Specificity: Natural and recombinant mouse DKK1