Mouse CLEC4F ELISA Kit PicoKine®, Carrier-free

• Background:

  C-type lectin domain family 4 member F is a protein that in humans is encoded by the CLEC4F gene. This gene is mapped to 6; 6 C3. CLEC4F is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily. Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain that contains an extended stalk region plus one carbohydrate recognition domain. Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation. CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues. CLEC4F preferentially binds galactose and N?acetylgalactosamine in a calcium-dependent manner. Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system.

• :

  heparin, EDTA or citrate

• Description:

  Mouse CLEC4F ELISA Kit PicoKine® (96 Tests). Quantitate Mouse CLEC4F in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	963	1121	1018	973	1018	62.56	6.1%
  Sample 2	3961	3775	4002	3504	3810	196.54	5.1%
  Sample 3	15064	17340	17384	14672	16115	1254.77	7.7%

  *number of samples for each test n=16.

• Synonyms:

  C-type lectin domain family 4 member F; C-type lectin superfamily member 13; C-type lectin 13; CLEC4F; CLECSF13

• Gene Name:

  C-type lectin domain family 4, member f

• Gene ID:

  51811

• UniProt:

  Q8N1N0

• Reactivity:

  Mouse

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: R70-G548

• Applications:

  ELISA

• Standard:

  40 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

• Detection Range:

  625 pg/ml - 40,000 pg/ml

• Sensitivity:

  <300 pg/ml

• Function:

  Receptor with an affinity for galactose and fucose. Could be involved in endocytosis.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Specificity:

  Natural and recombinant mouse CLEC4F

• Subcellular Location:

  Membrane. Single-pass type II membrane protein.