Human Plexin B2/PLXNB2 ELISA Kit PicoKine®, Carrier-free

• Background:

  Members of the B class of plexins, such as PLXNB2 are transmembrane receptors that participate in axon guidance and cell migration in response to semaphorins.This gene is mapped to 22q13.33.

• :

  heparin or EDTA

• Description:

  Human Plexin B2 ELISA Kit PicoKine® (96 Tests). Quantitate Human PLXNB2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	1214	1018	1165	1017	1103	87.72	7.9%
  Sample 2	4275	4570	4511	4741	4524	166.86	3.6%
  Sample 3	19603	18628	19571	18283	19021	578.86	3.0%

  *number of samples for each test n=16.

• Synonyms:

  Plexin-B2; MM1; PLXNB2; KIAA0315

• Gene Name:

  plexin B2

• Gene ID:

  23654

• UniProt:

  O15031

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: L20-L1838

• Applications:

  ELISA

• Standard:

  40 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin, EDTA).

• Detection Range:

  0.624 ng/ml - 40 ng/ml

• Sensitivity:

  <0.1 ng/ml

• Function:

  Cell surface receptor for SEMA4C, SEMA4D and SEMA4G that plays an important role in cell-cell signaling. Binding to class 4 semaphorins promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal differentiation

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Specificity:

  Natural and recombinant human Plexin B2

• Subcellular Location:

  Cell membrane, Single-pass type I membrane protein