Monkey primate TGF Alpha ELISA Kit PicoKine®, Carrier-free

Monkey primate TGF Alpha ELISA Kit PicoKine®, Carrier-free

• Background: Transforming growth factor alpha (TGF-alpha) is upregulated in some monkey primate cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene (TGFA) maps to monkey primate chromosome 2 close to the breakpoint of the t (2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic monkey primate TGF-alpha showed similar immunoreactivity when compared with monkey primate TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.
• Description: Monkey primate TGF Alpha ELISA Kit PicoKine® (96 Tests). Quantitate Monkey TGFA in cell culture supernatants, serum, plasma (heparin, EDTA) and monkey primate milk. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Protransforming growth factor alpha;Transforming growth factor alpha;TGF-alpha;EGF-like TGF;ETGF;TGF type 1;TGFA;
• Gene Name: Protransforming growth factor alpha
• Gene ID: 613031
• UniProt: P01135
• Reactivity: Monkey
• Cross Reactivity: There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: V40-A89
• Tissue Specificity: Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. .
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, serum, plasma (heparin, EDTA) and monkey primate milk.
• Detection Range: 15.6 pg/ml - 1,000 pg/ml
• Sensitivity: <1 pg/ml
• Function: TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• : heparin or EDTA
• Calculated Molecular Weight: 17006 MW
• Subcellular Location: Transforming growth factor alpha: Secreted, extracellular space.
• Protein Name: Protransforming growth factor alpha