Mouse Cripto/TDGF1 ELISA Kit PicoKine®, Carrier-free

Mouse Cripto/TDGF1 ELISA Kit PicoKine®, Carrier-free

• Background: Teratocarcinoma-derived growth factor 1 is a protein that in humans is encoded by the TDGF1 gene. It is mapped to 3p23-p21. The protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic developmentand tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants.
• Description: Mouse Cripto/TDGF1 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Tdgf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Teratocarcinoma-derived growth factor;Cripto growth factor;Epidermal growth factor-like Cripto protein;Tdgf1;Cripto;
• Gene Name: Teratocarcinoma-derived growth factor
• Gene ID: 21667
• UniProt: P51865
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: R26-Q150
• Tissue Specificity: Expressed at low level in specific organs of the adult animal such as spleen, heart, lung and brain. During gastrulation, expressed in the forming mesoderm. In later stages of the developing heart, expression is restricted to the truncus arteriosus.
• Applications: ELISA
• Standard: 10 ng/tube
• Sample Type: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
• Detection Range: 62.5 pg/ml - 4,000 pg/ml
• Sensitivity: <10 pg/ml
• Function: Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm.
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Specificity: Natural and recombinant mouse Cripto
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	110	110	114	115	112	2.27	2%
  Sample 2	661	633	642	685	655	19.92	3%
  Sample 3	1213	1047	1166	1231	1164	71.73	6.1%

  *number of samples for each test n=16.
• : heparin or EDTA
• Calculated Molecular Weight: 18754 MW
• Subcellular Location: Cell membrane ; Lipid-anchor, GPI-anchor .
• Protein Name: Teratocarcinoma-derived growth factor