Mouse IL7R/CD127 ELISA Kit PicoKine®, Carrier-free

Mouse IL7R/CD127 ELISA Kit PicoKine®, Carrier-free

• Background: The interleukin-7 receptor (IL7R) is a protein found on the surface of cells. It is mapped to 5p13.2. The protein encoded by this gene is a receptor for interleukine 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukine 2, 4, 7, 9, and 15. IL7R has been shown to play a critical role in the development of immune cells called lymphocytes-specifically in a process known as V (D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. What's more, IL7R antagonism is efficacious in treatment of EAE through its effects on Th17 cells and is a potential treatment for MS.
• Description: Mouse IL7R/CD127 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il7r in cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
• Synonyms: Interleukin-7 receptor subunit alpha;IL-7 receptor subunit alpha;IL-7R subunit alpha;IL-7R-alpha;IL-7RA;CD127;Il7r;
• Gene Name: Interleukin-7 receptor subunit alpha
• Gene ID: 16197
• UniProt: P16872
• Reactivity: Mouse
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.
• Immunogen: Expression system for standard: NS0; Immunogen sequence: E21-D239
• Tissue Specificity: Spleen, thymus and fetal liver.
• Applications: ELISA
• Standard: 20 ng/tube
• Sample Type: cell culture supernatants and serum.
• Detection Range: 31.2 pg/ml - 2,000 pg/ml
• Sensitivity: <10 pg/ml
• Function: Receptor for interleukin-7. Also acts as a receptor for thymic stromal lymphopoietin (TSLP).
• Components: Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4
• Storage Conditions: Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
• Calculated Molecular Weight: 51605 MW
• Specificity: Natural and recombinant mouse IL7R
• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	66	77	65	68	69	4.74	6.8%
  Sample 2	270	257	287	288	275	12.85	4.6%
  Sample 3	964	912	900	813	897	54.26	6%

  *number of samples for each test n=16.
• Subcellular Location: Membrane; Single-pass type I membrane protein.
• Sequence Similarities: Belongs to the type I cytokine receptor family. Type 4 subfamily.
• Protein Name: Interleukin-7 receptor subunit alpha