Human IL-18/IL-1F4/Interleukin-18 ELISA Kit PicoKine®, Carrier-free

• Background:

  Interleukin (IL)-18, also called Interferon-gamma-inducing factor (IGIF), augments natural killer (NK) activity in spleen cells. The gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids.1 IL-18 is a recently discovered cytokine that modulates both T helper type 1 (Th1) and Th2 responses.2 IL-18 is a potent proinflammatory cytokine with potential atherogenic properties. It is highly expressed in the atherosclerotic plaques compared with control normal arteries and is localized mainly in plaque macrophages.3

• :

  heparin or EDTA

• Description:

  Human IL-18 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL18 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	33	32	33	31	32	0.82	2.5%
  Sample 2	247	212	221	232	228	13.05	5.7%
  Sample 3	520	533	562	546	478	31.15	5.4%

  *number of samples for each test n=16.

• Synonyms:

  Interleukin-18;IL-18;Iboctadekin;Interferon gamma-inducing factor;IFN-gamma-inducing factor;Interleukin-1 gamma;IL-1 gamma;IL18;IGIF, IL1F4;

• Gene Name:

  Interleukin-18

• Gene ID:

  3606

• UniProt:

  Q14116

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: Y33-D197

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <1 pg/ml

• Function:

  Augments natural killer cell activity in spleen cells and stimulates interferon gamma production in T-helper type I cells.

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  22326 MW

• Specificity:

  Natural and recombinant human IL-18

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Belongs to the IL-1 family.

• Protein Name:

  Interleukin-18